Peptide Reconstitution Calculator
Enter your vial size, BAC water volume, and dose – get your exact draw volume, concentration, and days supply instantly.
Peptide Reconstitution Calculator
Never guess your dosing again. Enter your vial size, BAC water volume, and desired dose — we’ll calculate your exact injection volume, concentration, and total doses per vial.
- Injection volume in mL and units
- Concentration per mL
- Total doses and days supply
- Works with any peptide or vial size
Peptide Reconstitution Calculator
Select your syringe, vial size, BAC water, and desired dose to get your exact draw amount.
Always follow your research protocol guidelines.
How to use this calculator
Step 1 — Select Your Syringe Choose the insulin syringe size you’re working with: 0.3 mL (30 units), 0.5 mL (50 units), or 1.0 mL (100 units). This tells the calculator how to express your draw volume in syringe units so you can measure accurately.
Step 2 — Enter Your Vial Size and BAC Water Volume Select the total peptide quantity in your vial (in mg) and how much bacteriostatic water you’re adding. Most researchers use 1–2 mL of BAC water per vial. The ratio of these two numbers determines your concentration.
Step 3 — Set Your Per-Injection Dose Choose your dose in mg. The calculator instantly returns your draw volume in mL, the exact units to pull on your syringe, your concentration per mL, total doses in the vial, and your days supply.
How to Reconstitute Peptides
econstitution is the process of dissolving a lyophilized (freeze-dried) peptide powder into a liquid solution so it can be drawn and injected. Most peptide vials arrive as a dry powder — they must be reconstituted before use.
What you need: The peptide vial, bacteriostatic water (BAC water), an insulin syringe, and alcohol swabs.
Step 1 — Clean both stoppers. Wipe the rubber stopper on your BAC water vial and your peptide vial with an alcohol swab. Let them dry for 30 seconds.
Step 2 — Draw your BAC water. Using a fresh syringe, pull the desired volume of BAC water (typically 1–2 mL). Aim the needle at the side wall of the vial — not directly onto the powder — to avoid damaging the peptide.
Step 3 — Inject slowly. Slowly depress the plunger and let the water run down the side of the vial. Do not shake. Gently swirl or roll the vial between your fingers until the powder fully dissolves. This usually takes 30–60 seconds.
Step 4 — Store correctly. Once reconstituted, store your vial in the refrigerator (2–8°C). Most peptides remain stable for 4–6 weeks when stored properly. Do not freeze a reconstituted vial.
Use the calculator above to determine exactly how much to draw per injection based on your concentration.
Frequently Asked Questions
Q: What is bacteriostatic water and why do I need it? Bacteriostatic water (BAC water) is sterile water preserved with 0.9% benzyl alcohol, which prevents bacterial growth. It’s the standard diluent for peptide reconstitution because it keeps your solution stable in the refrigerator for weeks. Do not use plain sterile water — it has no preservative and the solution will degrade much faster.
Q: What syringe size should I use for peptide injections? Most researchers use U-100 insulin syringes in 0.3 mL, 0.5 mL, or 1.0 mL sizes. Smaller syringes (0.3 mL / 30-unit) are ideal for low-volume, precise draws. A 1.0 mL syringe works fine for larger draw volumes. Use the calculator above to determine which syringe size aligns best with your dose.
Q: How much BAC water should I add to my vial? The most common approach is 1–2 mL per vial. Adding less BAC water increases your concentration (smaller draw volume per dose). Adding more decreases concentration (larger draw volume). Neither is wrong — it’s a matter of preference and your syringe size. The calculator handles any BAC water volume.
Q: How long does a reconstituted peptide vial last? Most reconstituted peptides remain stable for 4–6 weeks when refrigerated at 2–8°C. Stability varies by peptide — some are more fragile than others. Always check your specific research protocol. Never freeze a reconstituted vial; freeze-thaw cycles degrade the peptide.
Q: What does “concentration” mean in the results? Concentration is expressed as mg per mL (mg/mL). It tells you how much peptide is dissolved in each milliliter of your solution. If you add 1 mL of BAC water to a 5 mg vial, your concentration is 5 mg/mL. This number is the basis for all dosing math — the calculator derives your draw volume directly from it.
Q: Can I use this calculator for any peptide? Yes. The math is the same regardless of the peptide — BPC-157, TB-500, Semaglutide, CJC-1295, Ipamorelin, or anything else. The calculator doesn’t care what’s in the vial, only the quantities: vial size, BAC water volume, and your target dose.
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